The publications of the members of the research group.
2023
Caccavo, Diego; Iannone, Marco; Barba, Anna Angela; Lamberti, Gaetano
Impact of drug release in USP II and in-vitro stomach on pharmacokinetic: The case study of immediate-release carbamazepine tablets Journal Article
In: Chemical Engineering Science, vol. 267, 2023.
Abstract | Links | BibTeX | Tags: Drug Delivery Systems, Mathematical modeling, Pharmacokinetics
@article{Caccavo2023,
title = {Impact of drug release in USP II and in-vitro stomach on pharmacokinetic: The case study of immediate-release carbamazepine tablets},
author = {Diego Caccavo and Marco Iannone and Anna Angela Barba and Gaetano Lamberti},
url = {https://www.sciencedirect.com/science/article/pii/S0009250922009563},
doi = {10.1016/j.ces.2022.118371},
year = {2023},
date = {2023-03-05},
journal = {Chemical Engineering Science},
volume = {267},
abstract = {The in-vitro reproduction of the real physiological conditions that occur along the gastrointestinal (GI) tract would be the optimum for the dissolution and release testing of pharmaceutical formulations for oral intake. In this study a method for the automated reproduction of the real pH conditions that occurs in the gastric cavity and a device that mimics the same forces exerted by the internal walls of the stomach are presented. Commercial immediate-release carbamazepine tablets were tested in conventional (USP II) and unconventional apparatuses. The gastric pH and the fluid dynamic conditions are factors to be carefully considered since they both affect the drug release profiles. Finally, a PBPK model was used to predict the evolution of plasma drug concentrations knowing the experimental in-vitro GI release behavior. It was found that, for immediate-release carbamazepine tablet, the gastric drug release does not have a major impact on the plasmatic drug concentration.},
keywords = {Drug Delivery Systems, Mathematical modeling, Pharmacokinetics},
pubstate = {published},
tppubtype = {article}
}
The in-vitro reproduction of the real physiological conditions that occur along the gastrointestinal (GI) tract would be the optimum for the dissolution and release testing of pharmaceutical formulations for oral intake. In this study a method for the automated reproduction of the real pH conditions that occurs in the gastric cavity and a device that mimics the same forces exerted by the internal walls of the stomach are presented. Commercial immediate-release carbamazepine tablets were tested in conventional (USP II) and unconventional apparatuses. The gastric pH and the fluid dynamic conditions are factors to be carefully considered since they both affect the drug release profiles. Finally, a PBPK model was used to predict the evolution of plasma drug concentrations knowing the experimental in-vitro GI release behavior. It was found that, for immediate-release carbamazepine tablet, the gastric drug release does not have a major impact on the plasmatic drug concentration.
2022
Caccavo, Diego; Vecchia, Marica Della; Barba, Anna Angela; Lamberti, Gaetano
Simil-microfluidic ethanol injection mixer for the continuous synthesis production of liposomes: laminar vs turbulent regime Proceedings Article
In: CHISA - Prague (Czech Republic), 2022.
BibTeX | Tags: Drug Delivery Systems, Micro and Nano Vectors
@inproceedings{Caccavo2022,
title = {Simil-microfluidic ethanol injection mixer for the continuous synthesis production of liposomes: laminar vs turbulent regime},
author = {Diego Caccavo and Marica {Della Vecchia} and Anna Angela Barba and Gaetano Lamberti},
year = {2022},
date = {2022-08-23},
booktitle = {CHISA - Prague (Czech Republic)},
keywords = {Drug Delivery Systems, Micro and Nano Vectors},
pubstate = {published},
tppubtype = {inproceedings}
}
2020
Lamberti, Gaetano; Barba, Anna Angela
Drug Delivery of siRNA Therapeutics Journal Article
In: Pharmaceutics, vol. 12(2), no. 178, 2020.
Links | BibTeX | Tags: aptamers, Drug Delivery Systems, gene therapy, liposomes, nanoparticles, polycations, siRNA
@article{Lamberti2020,
title = {Drug Delivery of siRNA Therapeutics},
author = {Gaetano Lamberti and Anna Angela Barba},
url = {https://www.mdpi.com/1999-4923/12/2/178/pdf},
doi = {10.3390/pharmaceutics12020178},
year = {2020},
date = {2020-02-20},
journal = {Pharmaceutics},
volume = {12(2)},
number = {178},
keywords = {aptamers, Drug Delivery Systems, gene therapy, liposomes, nanoparticles, polycations, siRNA},
pubstate = {published},
tppubtype = {article}
}
2019
Dalmoro, Annalisa; Bochicchio, Sabrina; Lamberti, Gaetano; Bertoncin, Paolo; Janssens, Barbara; Barba, Anna Angela
Micronutrients encapsulation in enhanced nanoliposomal carriers by a novel preparative technology Journal Article
In: RSC Advances, vol. 9, pp. 19800-19812, 2019.
Abstract | Links | BibTeX | Tags: Drug Delivery Systems, Micro and Nano Vectors
@article{Dalmoro2019,
title = {Micronutrients encapsulation in enhanced nanoliposomal carriers by a novel preparative technology },
author = {Annalisa Dalmoro and Sabrina Bochicchio and Gaetano Lamberti and Paolo Bertoncin and Barbara Janssens and Anna Angela Barba},
url = {https://pubs.rsc.org/en/content/articlelanding/2019/ra/c9ra03022k},
doi = {10.1039/C9RA03022K},
year = {2019},
date = {2019-06-25},
journal = {RSC Advances},
volume = {9},
pages = {19800-19812},
abstract = {Micronutrients administration by fortification of staple and complementary foods is a followed strategy to fight malnutrition and micronutrient deficiencies and related pathologies. There is a great industrial interest in preparation of formulations for joint administration of vitamin D3 and vitamin K2 for providing bone support, promoting heart health and helping boost immunity. To respond to this topic, in this work, uncoated nanoliposomes loaded with vitamin D3 and K2 were successfully prepared, by using a novel, high-yield and semi continuous technique based on simil-microfluidic principles. By the same technique, to promote and to enhance mucoadhesiveness and stability of the produced liposomal structures, chitosan was tested as covering material. By this way polymer\textendashlipid hybrid nanoparticles, encapsulating vitamin D3 and vitamin K2, with improved features in terms of stability, loading and mucoadhesiveness were produced for potential nutraceutical and pharmaceutical applications.},
keywords = {Drug Delivery Systems, Micro and Nano Vectors},
pubstate = {published},
tppubtype = {article}
}
Micronutrients administration by fortification of staple and complementary foods is a followed strategy to fight malnutrition and micronutrient deficiencies and related pathologies. There is a great industrial interest in preparation of formulations for joint administration of vitamin D3 and vitamin K2 for providing bone support, promoting heart health and helping boost immunity. To respond to this topic, in this work, uncoated nanoliposomes loaded with vitamin D3 and K2 were successfully prepared, by using a novel, high-yield and semi continuous technique based on simil-microfluidic principles. By the same technique, to promote and to enhance mucoadhesiveness and stability of the produced liposomal structures, chitosan was tested as covering material. By this way polymer–lipid hybrid nanoparticles, encapsulating vitamin D3 and vitamin K2, with improved features in terms of stability, loading and mucoadhesiveness were produced for potential nutraceutical and pharmaceutical applications.
2017
Kazlauske, Jurgita; Cafaro, Maria Margherita; Caccavo, Diego; Marucci, Maria Grazia; Lamberti, Gaetano; Barba, Anna Angela; Larsson, Anette
Determination of the release mechanism of Theophylline from pellets coated with Surelease® − a water dispersion of Ethyl cellulose Journal Article
In: International Journal of Pharmaceutics, vol. 528, no. 1-2, pp. 345-353, 2017, ISSN: 0378-5173.
Abstract | Links | BibTeX | Tags: Drug Delivery Systems, drug release, Hydrogel Characterization
@article{Kazlauske2017,
title = {Determination of the release mechanism of Theophylline from pellets coated with Surelease® − a water dispersion of Ethyl cellulose},
author = {Jurgita Kazlauske and Maria Margherita Cafaro and Diego Caccavo and Maria Grazia Marucci and Gaetano Lamberti and Anna Angela Barba and Anette Larsson},
url = {http://www.sciencedirect.com/science/article/pii/S0378517317304970},
doi = {10.1016/j.ijpharm.2017.05.073},
issn = {0378-5173},
year = {2017},
date = {2017-06-17},
journal = {International Journal of Pharmaceutics},
volume = {528},
number = {1-2},
pages = {345-353},
abstract = {The aim of this study was to investigate the water transport over free standing films based on the aqueous ethyl cellulose (EC) coating Surelease® and the drug (Theophylline) release mechanism from coated pellets. It was found that the main drug release rate from pellets was controlled by a diffusion mechanism. However, the drug release rate was altered by addition of sodium chloride to the external release medium. A decrease in the drug release rate when sodium chloride is added to the release medium has traditionally been used to indicate an osmotic drug release mechanism. However, our findings that the release rate decreased by sodium chloride addition could be explained by sodium chloride diffusing through the coating layer into the inner parts of the pellets, decreasing the solubility of Theophylline. This gave a reduced drug concentration gradient over the coating layer and thus a slower release rate. Furthermore, this study shows, as expected, that the transport of water through Surelease® films into the pellets was faster than the transport out of Theophylline (approx. seven times), which was the reason why the pellets were swelling during the release. It was also shown that the drug release rate, determined for both whole dose release and for single pellets, decreased with increasing thickness (from 16 to 51 μm) of the coating layer controlling the drug release rate.},
keywords = {Drug Delivery Systems, drug release, Hydrogel Characterization},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to investigate the water transport over free standing films based on the aqueous ethyl cellulose (EC) coating Surelease® and the drug (Theophylline) release mechanism from coated pellets. It was found that the main drug release rate from pellets was controlled by a diffusion mechanism. However, the drug release rate was altered by addition of sodium chloride to the external release medium. A decrease in the drug release rate when sodium chloride is added to the release medium has traditionally been used to indicate an osmotic drug release mechanism. However, our findings that the release rate decreased by sodium chloride addition could be explained by sodium chloride diffusing through the coating layer into the inner parts of the pellets, decreasing the solubility of Theophylline. This gave a reduced drug concentration gradient over the coating layer and thus a slower release rate. Furthermore, this study shows, as expected, that the transport of water through Surelease® films into the pellets was faster than the transport out of Theophylline (approx. seven times), which was the reason why the pellets were swelling during the release. It was also shown that the drug release rate, determined for both whole dose release and for single pellets, decreased with increasing thickness (from 16 to 51 μm) of the coating layer controlling the drug release rate.
Caccavo, Diego; Lamberti, Gaetano; Cafaro, Maria Margherita; Barba, Anna Angela; Kazlauske, Jurgita; Larsson, Anette
Mathematical modeling of the drug release from an ensemble of coated pellets Journal Article
In: British Journal of Pharmacology, vol. 174, no. 12, pp. 1797–1809 , 2017, ISBN: 1476-5381.
Abstract | Links | BibTeX | Tags: Drug Delivery Systems, drug release, Hydrogel Characterization, Hydrogel Modeling
@article{Caccavo2017b,
title = {Mathematical modeling of the drug release from an ensemble of coated pellets},
author = {Diego Caccavo and Gaetano Lamberti and Maria Margherita Cafaro and Anna Angela Barba and Jurgita Kazlauske and Anette Larsson},
url = {http://onlinelibrary.wiley.com/doi/10.1111/bph.13776/abstract},
doi = {10.1111/bph.13776},
isbn = {1476-5381},
year = {2017},
date = {2017-04-22},
journal = {British Journal of Pharmacology},
volume = {174},
number = {12},
pages = {1797\textendash1809 },
abstract = {Background and Purpose
Coated pellets are widely used as oral drug delivery systems, being highly accepted by patients and with several advantages with respect to single unit devices. The understanding of their behavior is therefore needed to improve the formulation effectiveness and to reduce the production costs. In spite of such an importance, not many mathematical modeling attempts have been made, mostly due to the complexities arising from the system polydispersity (non homogeneous multiple-unit particulate systems), which has been scarcely investigated with the aid of mechanistic models.
Experimental approach
In this work a mechanistic mathematical model able to describe the single pellet behavior in terms of hydration, drug dissolution, diffusion and release, and particle size change was developed. This model was then extended to describe and predict the behavior of mono- and poly-disperse ensembles of pellets.
Key Results
In particular the polydispersity arising from the inert core size distribution was proved to have a minimal effect on the drug release profile, whereas the size distribution of the polymeric film thickness showed to be the key parameter determining the drug release.
Conclusions and Implications
The developed mechanistic model, capable of considering the polydispersity of the system, was able to predict the release kinetics from ensembles of pellets and to highlight the key parameters to control in the production of pellets-based drug delivery systems, demonstrating its use as a powerful predictive tool.},
keywords = {Drug Delivery Systems, drug release, Hydrogel Characterization, Hydrogel Modeling},
pubstate = {published},
tppubtype = {article}
}
Background and Purpose
Coated pellets are widely used as oral drug delivery systems, being highly accepted by patients and with several advantages with respect to single unit devices. The understanding of their behavior is therefore needed to improve the formulation effectiveness and to reduce the production costs. In spite of such an importance, not many mathematical modeling attempts have been made, mostly due to the complexities arising from the system polydispersity (non homogeneous multiple-unit particulate systems), which has been scarcely investigated with the aid of mechanistic models.
Experimental approach
In this work a mechanistic mathematical model able to describe the single pellet behavior in terms of hydration, drug dissolution, diffusion and release, and particle size change was developed. This model was then extended to describe and predict the behavior of mono- and poly-disperse ensembles of pellets.
Key Results
In particular the polydispersity arising from the inert core size distribution was proved to have a minimal effect on the drug release profile, whereas the size distribution of the polymeric film thickness showed to be the key parameter determining the drug release.
Conclusions and Implications
The developed mechanistic model, capable of considering the polydispersity of the system, was able to predict the release kinetics from ensembles of pellets and to highlight the key parameters to control in the production of pellets-based drug delivery systems, demonstrating its use as a powerful predictive tool.
Coated pellets are widely used as oral drug delivery systems, being highly accepted by patients and with several advantages with respect to single unit devices. The understanding of their behavior is therefore needed to improve the formulation effectiveness and to reduce the production costs. In spite of such an importance, not many mathematical modeling attempts have been made, mostly due to the complexities arising from the system polydispersity (non homogeneous multiple-unit particulate systems), which has been scarcely investigated with the aid of mechanistic models.
Experimental approach
In this work a mechanistic mathematical model able to describe the single pellet behavior in terms of hydration, drug dissolution, diffusion and release, and particle size change was developed. This model was then extended to describe and predict the behavior of mono- and poly-disperse ensembles of pellets.
Key Results
In particular the polydispersity arising from the inert core size distribution was proved to have a minimal effect on the drug release profile, whereas the size distribution of the polymeric film thickness showed to be the key parameter determining the drug release.
Conclusions and Implications
The developed mechanistic model, capable of considering the polydispersity of the system, was able to predict the release kinetics from ensembles of pellets and to highlight the key parameters to control in the production of pellets-based drug delivery systems, demonstrating its use as a powerful predictive tool.
2015
Barba, Anna Angela; Lamberti, Gaetano; Sardo, Carla; Dapas, Barbara; Abrami, Michela; Grassi, Mario; Farra, Rossella; Tonon, F; Forte, Giancarlo; Musiani, F; Licciardi, M; Pozzato, G; Zanconati, F; Scaggiante, Bruna; Grassi, Gabriele; Cavallaro, Gennara
Novel Lipid and Polymeric Materials As Delivery Systems for Nucleic Acid Based Drugs. Journal Article
In: Current drug metabolism, vol. 16, no. 6, pp. 427-452, 2015, ISSN: 1389-2002.
Abstract | Links | BibTeX | Tags: Biopolymer, Drug Delivery Systems, liposome, Micro and Nano Vectors, nucleic acid based drugs
@article{Barba2015,
title = {Novel Lipid and Polymeric Materials As Delivery Systems for Nucleic Acid Based Drugs.},
author = { Anna Angela Barba and Gaetano Lamberti and Carla Sardo and Barbara Dapas and Michela Abrami and Mario Grassi and Rossella Farra and F Tonon and Giancarlo Forte and F Musiani and M Licciardi and G Pozzato and F Zanconati and Bruna Scaggiante and Gabriele Grassi and Gennara Cavallaro},
url = {http://benthamscience.com/journals/current-drug-metabolism/article/133927/},
doi = {10.2174/1389200216666150812142557},
issn = {1389-2002},
year = {2015},
date = {2015-01-01},
journal = {Current drug metabolism},
volume = {16},
number = {6},
pages = {427-452},
abstract = {Nucleic acid based drugs (NADBs) are short DNA/RNA molecules that include among others, antisense oligonucleotides, aptamers, small interfering RNAs and micro-interfering RNAs. Despite the different mechanisms of actions, NABDs have the ability to combat the effects of pathological gene expression in many experimental systems. Thus, nowadays, NABDs are considered to have a great therapeutic potential, possibly superior to that of available drugs. Unfortunately, however, the lack of effective delivery systems limits the practical use of NABDs. Due to their hydrophilic nature, NABDs cannot efficiently cross cellular membrane; in addition, they are subjected to fast degradation by cellular and extracellular nucleases. Together these aspects make the delivery of NABDs as naked molecules almost un-effective. To optimize NABD delivery, several solutions have been investigated. From the first attempts described in the beginning of the 1980s, a burst in the number of published papers occurred in the beginning of 1990s reaching a peak in 2012-13. The extensive amount of work performed so far clearly witnesses the interest of the scientific community in this topic. In the present review, we will concentrate on the description of the most interesting advances in the field. Particular emphasis will be put on polymeric and lipid materials used alone or in combination with a promising delivery strategy based on the use of carbon nanotubes. The data presented suggest that, although further improvements are required, we are not far from the identification of effective delivery systems for NABDs thus making the clinical use of these molecules closer to reality.},
keywords = {Biopolymer, Drug Delivery Systems, liposome, Micro and Nano Vectors, nucleic acid based drugs},
pubstate = {published},
tppubtype = {article}
}
Nucleic acid based drugs (NADBs) are short DNA/RNA molecules that include among others, antisense oligonucleotides, aptamers, small interfering RNAs and micro-interfering RNAs. Despite the different mechanisms of actions, NABDs have the ability to combat the effects of pathological gene expression in many experimental systems. Thus, nowadays, NABDs are considered to have a great therapeutic potential, possibly superior to that of available drugs. Unfortunately, however, the lack of effective delivery systems limits the practical use of NABDs. Due to their hydrophilic nature, NABDs cannot efficiently cross cellular membrane; in addition, they are subjected to fast degradation by cellular and extracellular nucleases. Together these aspects make the delivery of NABDs as naked molecules almost un-effective. To optimize NABD delivery, several solutions have been investigated. From the first attempts described in the beginning of the 1980s, a burst in the number of published papers occurred in the beginning of 1990s reaching a peak in 2012-13. The extensive amount of work performed so far clearly witnesses the interest of the scientific community in this topic. In the present review, we will concentrate on the description of the most interesting advances in the field. Particular emphasis will be put on polymeric and lipid materials used alone or in combination with a promising delivery strategy based on the use of carbon nanotubes. The data presented suggest that, although further improvements are required, we are not far from the identification of effective delivery systems for NABDs thus making the clinical use of these molecules closer to reality.
2014
Bochicchio, Sabrina; Dalmoro, Annalisa; Barba, Anna Angela; Grassi, Gabriele; Lamberti, Gaetano
Liposomes as siRNA Delivery Vectors Journal Article
In: Current drug metabolism, vol. 15, no. 9, pp. 882–892, 2014, ISSN: 1389-2002.
Abstract | Links | BibTeX | Tags: Drug Delivery Systems, liposome, Micro and Nano Vectors, siRNA
@article{Bochicchio2014,
title = {Liposomes as siRNA Delivery Vectors},
author = {Sabrina Bochicchio and Annalisa Dalmoro and Anna Angela Barba and Gabriele Grassi and Gaetano Lamberti},
url = {http://www.eurekaselect.com/128256/article},
doi = {10.2174/1389200216666150206124913},
issn = {1389-2002},
year = {2014},
date = {2014-01-01},
journal = {Current drug metabolism},
volume = {15},
number = {9},
pages = {882--892},
publisher = {Bentham Science Publishers},
abstract = {Nucleic Acid Based Drugs (NABDs) constitute a class of promising and powerful therapeutic new agents with limited side effects, potentially useable against a wide range of diseases, including cancer. Among them, the short interfering RNAs (siRNAs), represent very effective molecules. Despite their in vitro efficacy, the major drawback that limits siRNAs usage consists in a difficult delivery due to their very low stability in physiological fluids, and to their limited membrane-permeability through physiological barriers. On the other hand, the liposomes (lipid bilayers closed in vesicles of various sizes) represent interesting drug delivery systems (DDSs) which can be tailored in order to get the best performance in terms of load, vesicle size and transfection yield. In this work, the current state of study in these two fields, and the connections between them, are briefly summarized.},
keywords = {Drug Delivery Systems, liposome, Micro and Nano Vectors, siRNA},
pubstate = {published},
tppubtype = {article}
}
Nucleic Acid Based Drugs (NABDs) constitute a class of promising and powerful therapeutic new agents with limited side effects, potentially useable against a wide range of diseases, including cancer. Among them, the short interfering RNAs (siRNAs), represent very effective molecules. Despite their in vitro efficacy, the major drawback that limits siRNAs usage consists in a difficult delivery due to their very low stability in physiological fluids, and to their limited membrane-permeability through physiological barriers. On the other hand, the liposomes (lipid bilayers closed in vesicles of various sizes) represent interesting drug delivery systems (DDSs) which can be tailored in order to get the best performance in terms of load, vesicle size and transfection yield. In this work, the current state of study in these two fields, and the connections between them, are briefly summarized.
2011
Grassi, Mario; Lamberti, Gaetano; Cascone, Sara; Grassi, Gabriele
Mathematical modeling of simultaneous drug release and in vivo absorption. Journal Article
In: International journal of pharmaceutics, vol. 418, no. 1, pp. 130–41, 2011, ISSN: 1873-3476.
Abstract | Links | BibTeX | Tags: Absorption, Administration, Area Under Curve, Biological, Drug Delivery Systems, Humans, In silico, Models, Oral, Pharmacokinetics, Solubility, Theoretical
@article{Grassi2011,
title = {Mathematical modeling of simultaneous drug release and in vivo absorption.},
author = { Mario Grassi and Gaetano Lamberti and Sara Cascone and Gabriele Grassi},
url = {http://www.sciencedirect.com/science/article/pii/S0378517311000275},
doi = {10.1016/j.ijpharm.2010.12.044},
issn = {1873-3476},
year = {2011},
date = {2011-10-01},
journal = {International journal of pharmaceutics},
volume = {418},
number = {1},
pages = {130--41},
abstract = {The attention of this review is focussed on the mathematical modeling of the simultaneous processes of drug release and absorption/distribution/metabolism/elimination (ADME processes) following different administration routes. Among all of them, for their clinical importance, the oral, transdermal and local delivery are considered. The bases of the presented mathematical models are shown after the discussion of the most relevant phenomena characterising the particular administration route considered. Then, model performances are compared to experimental evidences in order to evaluate their reliability and soundness. The most important conclusion of this review is that despite the complexity of the problem involved in the description of the fate of the drugs after their administration, the scientific community is close to the solution as witnessed by the various interesting and promising approaches here presented about the oral, transdermal and local administration routes.},
keywords = {Absorption, Administration, Area Under Curve, Biological, Drug Delivery Systems, Humans, In silico, Models, Oral, Pharmacokinetics, Solubility, Theoretical},
pubstate = {published},
tppubtype = {article}
}
The attention of this review is focussed on the mathematical modeling of the simultaneous processes of drug release and absorption/distribution/metabolism/elimination (ADME processes) following different administration routes. Among all of them, for their clinical importance, the oral, transdermal and local delivery are considered. The bases of the presented mathematical models are shown after the discussion of the most relevant phenomena characterising the particular administration route considered. Then, model performances are compared to experimental evidences in order to evaluate their reliability and soundness. The most important conclusion of this review is that despite the complexity of the problem involved in the description of the fate of the drugs after their administration, the scientific community is close to the solution as witnessed by the various interesting and promising approaches here presented about the oral, transdermal and local administration routes.